Combined Effects of 528 Hz Sound and X-ray in Peripheral Blood Lymphocytes

Introduction: Radiotherapy is still one of the main options for cancer treatment but it is in association with damage to normal cells as well as the tumor cells. To reduce the injury in normal cells we have evaluated the effect of 528 hertz sound after X irradiation in peripheral blood lymphocytes. Materials and Methods: in this study, peripheral blood was obtained from 25 healthy volunteers and blood lymphocytes were separated and divided into 4 groups: Ι) Control group, П) Radiation group, Ш) Radiation + Sound group ΙV) Sound group. To measure radiation effect on DNA, nuclear (DNA) damage rate was evaluated in all groups by the use of the comet assay. Results: The obtained data revealed that DNA damage rate in radiation group (190±45) is significantly higher than control (137±32), Sound (152±29). But, the difference of DNA damage in control with sound and also radiation+sound groups was not statistically significant.   Conclusion: The results indicating that 528 hertz sound could induce radiation damage but when it will be combined with X radiation, the DNA damages of X-rays in peripheral blood lymphocytes is not significantly different with control groups which indicate modulation of DNA damages and addresses the needs for further studies.